The Potential Economic Returns of Converting Agricultural Land to Forestry: An Analysis of System and Soil Effects from 1995 to 2009

peer-reviewedPrivate land owners have been responsible for the majority of annual afforestation in Ireland since the mid1990s, but planting rates have generally been declining since 2002. Although the decision to plant may be driven by a number of factors, the profitability of forestry as a landuse option should be an important driver and offer some insight into trends in afforestation rates. As farmers undertake most afforestation in Ireland it is important to account for the opportunity cost of lost agricultural income when analysing the financial outcome of planting. In addition, soil quality plays an essential role in dictating the productivity and profitability of both agriculture and forestry. This study examines the effects of soil quality and superseded agricultural system on the potential profitability of afforestation by farmers between 1995 and 2009. Data from the National Farm Survey were employed to identify the annual gross margins for six agricultural systems on six soil types that differ in terms of quality. The measures of soil quality were translated into potential yield classes for forestry using an existing productivity model and Teagasc’s Forest Investment and Valuation Estimator was employed to calculate the net present value of afforestation for each of the systems and soil types. The results demonstrate how the competitiveness of forestry as a landuse option is influenced by soil quality and superseded enterprise and how forestry has become more competitive with agricultural enterprises over the period of analysis.Department of Agriculture, Food and the Marin

The role of extension and forest characteristics in understanding the management decisions of new forest owners in Ireland

peer-reviewedMany European countries have seen significant changes in forest ownership structure, with the emergence of a cohort commonly referred to as new forest owners, mainly within the non-industrial, private forest (NIPF) owner group. The drivers of this change differ between countries but these owners frequently lack an existing knowledge base to draw on regarding forest management decisions and practices and may possess different objectives to traditional owners. As a result there is uncertainty concerning the management intentions of these owners. The provision of extension services is a recognised approach to supporting decision-making by NIPF owners but there have been relatively few studies that have sought to quantify the effectiveness of such initiatives in terms of management outcomes. In addition to measuring the outcome of extension initiatives, exploring the positive or negative outcomes can assist with the design of future initiatives. Ensuring that such initiatives are designed for appropriate phases in the forest life-cycle is important. This paper reports the results from a number of surveys that sought to explore the impact of an extension initiative, a thinning demonstration, on actual management outcomes and what characteristics of owners and their forests might explain observed management decisions. A retrospective pre-post test questionnaire was used at the demonstration to capture knowledge impacts and management intentions. A follow up survey was conducted 18 months later to investigate what, if any, practices had been undertaken. Data from a national household survey of land owners were also analysed to investigate whether the observations from the demonstration had significance for the wider population. The results suggest that the demonstration was successful in imparting knowledge to forest owners both in terms of self-reported learning and actual management outcomes. However, from an Irish perspective management decisions are dominated by forest age as the majority of the private estate is still in its first rotation. This presents a challenge to extension service personnel and to research seeking to explain management practices at a national level

Epileptogenic effects of NMDAR antibodies in a passive transfer mouse model

Most patients with N-methyl D-aspartate-receptor antibody encephalitis develop seizures but the epileptogenicity of the antibodies has not been investigated in vivo. Wireless electroencephalogram transmitters were implanted into 23 C57BL/6 mice before left lateral ventricle injection of antibody-positive (test) or healthy (control) immunoglobulin G. Mice were challenged 48 h later with a subthreshold dose (40 mg/kg) of the chemo-convulsant pentylenetetrazol and events recorded over 1 h. Seizures were assessed by video observation of each animal and the electroencephalogram by an automated seizure detection programme. No spontaneous seizures were seen with the antibody injections. However, after the pro-convulsant, the test mice (n = 9) had increased numbers of observed convulsive seizures (P = 0.004), a higher total seizure score (P = 0.003), and a higher number of epileptic 'spike' events (P = 0.023) than the control mice (n = 6). At post-mortem, surprisingly, the total number of N-methyl D-aspartate receptors did not differ between test and control mice, but in test mice the levels of immunoglobulin G bound to the left hippocampus were higher (P < 0.0001) and the level of bound immunoglobulin G correlated with the seizure scores (R2 = 0.8, P = 0.04, n = 5). Our findings demonstrate the epileptogenicity of N-methyl D-aspartate receptor antibodies in vivo, and suggest that binding of immunoglobulin G either reduced synaptic localization of N-methyl D-aspartate receptors, or had a direct effect on receptor function, which could be responsible for seizure susceptibility in this acute short-term model

NMDA-receptor antibodies alter cortical microcircuit dynamics

NMDA-receptor antibodies (NMDAR-Abs) cause an autoimmune encephalitis with a diverse range of EEG abnormalities. NMDAR-Abs are believed to disrupt receptor function, but how blocking this excitatory synaptic receptor can lead to paroxysmal EEG abnormalities-or even seizures-is poorly understood. Here we show that NMDAR-Abs change intrinsic cortical connections and neuronal population dynamics to alter the spectral composition of spontaneous EEG activity and predispose brain dynamics to paroxysmal abnormalities. Based on local field potential recordings in a mouse model, we first validate a dynamic causal model of NMDAR-Ab effects on cortical microcircuitry. Using this model, we then identify the key synaptic parameters that best explain EEG paroxysms in pediatric patients with NMDAR-Ab encephalitis. Finally, we use the mouse model to show that NMDAR-Ab-related changes render microcircuitry critically susceptible to overt EEG paroxysms when these key parameters are changed, even though the same parameter fluctuations are tolerated in the in silico model of the control condition. These findings offer mechanistic insights into circuit-level dysfunction induced by NMDAR-Ab

Groundwater : the world's neglected defence against climate change

Currently, millions of people across the globe don’t have safe water to drink. As climate change continues to wreak havoc, communities will see their homes and means of survival washed away, their drinking water contaminated or dry up, their crops wither and fail, their health devastated by infectious diseases, and their children forced out of school. Communities need sustainable and safe water and sanitation to have the best chance of combatting the devastating impacts of extreme weather, like heatwaves, droughts and floods. Yet one in four people across the globe do not have safely managed water in their homes. However, new analysis by the British Geological Survey (BGS) and WaterAid, reveals that many countries in Africa – including most parts of subSaharan Africa – and parts of Asia, have enough water to meet everyone’s daily needs. And this hidden resource is often right under our feet – groundwater. Groundwater – which exists almost everywhere underground, in gaps within soil, sand and rock – has the potential to save hundreds of thousands of lives and be the world’s insurance policy against climate change. It would help communities cope with slow onset climate impacts like drought and irregular rainfall, and provide broader resilience after floods by ensuring there is safe water available for all. But groundwater will only be able to lessen the impacts of climate change if it is carefully managed and if we invest in mechanisms to ensure that it gets to the people who need it most. All too often, this is not the case. In some regions, there isn’t enough investment in the services needed to find, capture, treat, manage and distribute groundwater – so it remains largely untouched. In others, we see rampant over-extraction with far too much groundwater being used, particularly by the agricultural sector. In both cases, only a limited amount of this life-saving resource gets to those who need it most. BGS and WaterAid assessed data on the amount of groundwater there is, how quickly it is replenished by rain, and how much the rocks can store. Our experts concluded that, on a national level, most countries in Africa have sufficient groundwater for people to not only survive, but to thrive. This includes countries such as Ethiopia and Madagascar, where only half the population have clean water close to home, and large parts of Mali, Niger and Nigeria. Although, on a sub-national level, there are some places where groundwater is more difficult to get to or is contaminated, our research estimated that today’s total groundwater on the continent could provide people with enough drinking water for at least five years in the event of a drought – and in some cases even decades. This calculation is based on 130 litres of domestic water use a day per capita, which would provide people with more than enough to drink, cook and wash with.i What’s more, as groundwater is below the surface, it is more resilient to extreme weather than other water sources – such as lakes, rivers, streams and dams – and is largely protected from evaporation and less susceptible to pollution. This means that even if our weather becomes more extreme and unpredictable, there is enough groundwater stored in aquifersii to provide a buffer for many years to come for the millions of people living on the frontline of climate change. For them, daily life is already a struggle simply because they do not have access to sustainable and safe water and sanitation

Cellular location and activity of Escherichia coli RecG proteins shed light on the function of its structurally unresolved C-terminus

RecG is a DNA translocase encoded by most species of bacteria. The Escherichia coli protein targets branched DNA substrates and drives the unwinding and rewinding of DNA strands. Its ability to remodel replication forks and to genetically interact with PriA protein have led to the idea that it plays an important role in securing faithful genome duplication. Here we report that RecG co-localises with sites of DNA replication and identify conserved arginine and tryptophan residues near its C-terminus that are needed for this localisation. We establish that the extreme C-terminus, which is not resolved in the crystal structure, is vital for DNA unwinding but not for DNA binding. Substituting an alanine for a highly conserved tyrosine near the very end results in a substantial reduction in the ability to unwind replication fork and Holliday junction structures but has no effect on substrate affinity. Deleting or substituting the terminal alanine causes an even greater reduction in unwinding activity, which is somewhat surprising as this residue is not uniformly present in closely related RecG proteins. More significantly, the extreme C-terminal mutations have little effect on localisation. Mutations that do prevent localisation result in only a slight reduction in the capacity for DNA repair. © 2014 The Author(s)

Proteoglycan Breakdown of Meniscal Explants Following Dynamic Compression Using a Novel Bioreactor

Motivated by our interest in examining meniscal mechanotransduction processes, we report on the validation of a new tissue engineering bioreactor. This paper describes the design and performance capabilities of a tissue engineering bioreactor for cyclic compression of meniscal explants. We showed that the system maintains a tissue culture environment equivalent to that provided by conventional incubators and that its strain output was uniform and reproducible. The system incorporates a linear actuator and load cell aligned together in a frame that is contained within an incubator and allows for large loads and small displacements. A plunger with six Teflon-filled Delrin compression rods is attached to the actuator compressing up to six tissue explants simultaneously and with even pressure. The bioreactor system was used to study proteoglycan (PG) breakdown in porcine meniscal explants following various input loading tests (0–20% strain, 0–0.1 MPa). The greatest PG breakdown was measured following 20% compressive strain. These strain and stress levels have been shown to correspond to partial meniscectomy. Thus, these data suggest that removing 30–60% of meniscal tissue will result in the breakdown of meniscal tissue proteoglycans

NMDA-receptor antibodies alter cortical microcircuit dynamics

NMDA-receptor antibodies (NMDAR-Abs) cause an autoimmune encephalitis with a diverse range of EEG abnormalities. NMDAR-Abs are believed to disrupt receptor function, but how blocking this excitatory synaptic receptor can lead to paroxysmal EEG abnormalities-or even seizures-is poorly understood. Here we show that NMDAR-Abs change intrinsic cortical connections and neuronal population dynamics to alter the spectral composition of spontaneous EEG activity and predispose brain dynamics to paroxysmal abnormalities. Based on local field potential recordings in a mouse model, we first validate a dynamic causal model of NMDAR-Ab effects on cortical microcircuitry. Using this model, we then identify the key synaptic parameters that best explain EEG paroxysms in pediatric patients with NMDAR-Ab encephalitis. Finally, we use the mouse model to show that NMDAR-Ab-related changes render microcircuitry critically susceptible to overt EEG paroxysms when these key parameters are changed, even though the same parameter fluctuations are tolerated in the in silico model of the control condition. These findings offer mechanistic insights into circuit-level dysfunction induced by NMDAR-Ab

Horticulture Research

Soil microbiota has increasingly been shown to play an integral role in viticulture resilience. The emergence of new metagenomic and culturomic technologies has led to significant advances in the study of microbial biodiversity. In the agricultural sector, soil and plant microbiomes have been found to significantly improve resistance to environmental stressors and diseases, as well as influencing crop yields and fruit quality thus improving sustainability under shifting environments. Grapevines are usually cultivated as a scion grafted on rootstocks, which are selected according to pedoclimatic conditions and cultural practices, known as terroir. The rootstock connects the surrounding soil to the vine’s aerial part and impacts scion growth and berry quality. Understanding rootstock and soil microbiome dynamics is a relevant and important field of study, which may be critical to improve viticulture sustainability and resilience. This review aims to highlight the relationship between grapevine roots and telluric microbiota diversity and activity. In addition, this review explores the concept of core microbiome regarding potential applications of soil microbiome engineering with the goal of enhancing grapevine adaptation to biotic and abiotic stress